Module

Hands-on Industrial Training With
State of Art Lectures on Advanced

Module 3

"Hands-on Industrial Training with State of Art Lectures on Basic & Advanced Molecular Biology"

*(Program Code: CRT- 03).

Duration 30 Days / One Month 15 Days
Seats 15 10
Fee Structure Rs. 6,800/- Rs. 4,200/-

Techniques Details:(For Duration of 30 Days)

S.No Lab Schedule
1 Working in Molecular Biology Laboratory.
2 General and Safety Instructions.
3 Good Laboratory Practices.
4 Principle and Handling of Laboratory Equipments.
5 Basics of Calculations, Weighing and Measurements.
6 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
7 Process of Sterilization and Decontamination.
8 Extraction and Purification of Genomic DNA from Various Parts of Plants.
9 Electrophoresis of Genomic DNA.
10 Calculation of Yield of DNA per gram of Plant Material.
11 Determination of Contaminants and Concentration of DNA by Spectrophotometer.
12 Extraction and Purification of Genomic DNA from Microbes.
13 Electrophoresis of Genomic DNA.
14 Determination of Purity of Bacterial Genomic DNA.
15 Determination of Contaminants and Concentration of Genomic DNA by Spectrophotometer.
16 Extraction and Purification of Plasmid DNA from E. coli..
17 Electrophoresis of Plasmid DNA.
18 Determination of Purity of Plasmid DNA.
19 Determination of Contaminants and Concentration of Plasmid DNA by Spectrophotometer.
20 RNA Extraction from Plants.
21 Extraction and Purification of RNA from Various Plant Materials.
22 Denaturing Gel Electrophoresis of RNA.
23 Determination of Purity of RNA.
24 Southern Blotting for DNA.
25 Northern Blotting for RNA.
26 Restriction Digestion of DNA by Restriction Endonucleases.
27 Separation of Digestion Fragments by Electrophoresis.
28 Ligation of Restriction Fragments into Plasmid.
29 Competent Cell Preparation.
30 Transfer of DNA into E. coli.
31 Screening of the Transformed Cells.
31 Effect of Physical and Chemical Mutagen on Growth of Microbes.
32 Blue-White Selection of Bacterial Colonies.
33 Polymerase Chain Reaction (PCR).
34 Electrophoresis of PCR Products.

Techniques Details:(For Duration of 15 Days)

S.No Lab Schedule
1 Working in Molecular Biology Laboratory.
2 General and Safety Instructions.
3 Good Laboratory Practices.
4 Principle and Handling of Laboratory Equipments.
5 Basics of Calculations, Weighing and Measurements.
6 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
7 Process of Sterilization and Decontamination.
8 Extraction and Purification of Genomic DNA from Various Parts of Plants.
9 Electrophoresis of Genomic DNA.
10 Calculation of Yield of DNA per gram of Plant Material.
11 Determination of Contaminants and Concentration of DNA by Spectrophotometer.
12 Extraction and Purification of Genomic DNA from Microbes.
13 Electrophoresis of Genomic DNA.
14 Determination of Purity of Bacterial Genomic DNA.
15 Determination of Contaminants and Concentration of Genomic DNA by Spectrophotometer.
16 Extraction and Purification of Plasmid DNA from E. coli.
17 Electrophoresis of Plasmid DNA.
18 Determination of Purity of Plasmid DNA.Determination of Purity of Plasmid DNA.
19 Determination of Contaminants and Concentration of Plasmid DNA by Spectrophotometer.
20 Polymerase Chain Reaction (PCR).
21 Electrophoresis of PCR Products.

Module 4

"Hands-on Industrial Training with State of Art Lectures on Basic & Advanced Biochemical Techniques & Proteomics"

*(Program Code: CRT- 04).

Duration 30 Days / One Month 15 Days
Seats 15 10
Fee Structure Rs. 6,800/- Rs. 4,200/-

Techniques Details:(For Duration of 30 Days)

S.No Lab Schedule
1 General and Safety Instructions.
2 Good Laboratory Practices.
3 Principle and Handling of Laboratory Equipments.
4 Basics of Calculations, Weighing and Measurements.
5 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
6 Process of Sterilization and Decontamination.
7 Isolation of Proteins from Plants and Microbial Systems.
8 Extraction of Total Protein from Various Parts of Plants.
9 Partial Purification of Plant Proteins by Precipitation Method.
10 Calculation of Yield of Protein per gram of Plant Material by Bradford's Method.
11 Calculation of Yield of Protein per gram of Plant Material by Lowry's Method.
12 Separation and Characterisation of Various Proteins.
13 SDS-PAGE.
14 Western Blotting for Purified Proteins.
15 Extraction and Estimation of Bioactive Compounds from Various Plants.
16 Determination of Potency of Bioactive Compounds.
17 Extraction of Antibiotics from Active Microbes.
18 Determination of Efficacy and Potency of Antibiotics.
19 Fermentation.
20 Downstream Processing (DSP).
21 Salt Precipitation.
22 Dialysis.
23 Solvent Precipitation.
24 Paper Chromatography.
25 Column Chromatography.
26 Thin Layer Chromatography (TLC).

Techniques Details:(For Duration of 15 Days)

S.No Lab Schedule
1 General and Safety Instructions.
2 Good Laboratory Practices.
3 Principle and Handling of Laboratory Equipments.
4 Basics of Calculations, Weighing and Measurements.
5 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
6 Process of Sterilization and Decontamination.
7 Isolation of Proteins from Plants and Microbial Systems.
8 Extraction of Total Protein from Various Parts of Plants.
9 Partial Purification of Plant Proteins by Precipitation Method.
10 Calculation of Yield of Protein per gram of Plant Material by Bradford's Method.
11 SDS-PAGE.
12 Extraction and Estimation of Bioactive Compounds from Various Plants.
13 Determination of Potency of Bioactive Compounds.
14 Solvent Precipitation.
15 Paper Chromatography.
16 Thin Layer Chromatography (TLC).

Module 5

"Hands-on Industrial Training with State of Art Lectures on Enzymology"

*(Program Code: CRT- 05).

Duration 30 Days / One Month 15 Days
Seats 15 10
Fee Structure Rs. 6,800/- Rs. 4,200/-

Techniques Details:(For Duration of 30 Days)

S.No Lab Schedule
1 General and Safety Instructions.
2 Good Laboratory Practices.
3 Principle and Handling of Laboratory Equipments.
4 Basics of Calculations, Weighing and Measurements.
5 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
6 Process of Sterilization and Decontamination.
7 Introduction to Enzymology.
8 Pure Culture Preparation.
9 Screening of Putative Cultures for Enzyme Production.
10 Fermentation (Submerged & Solid State).
11 Downstream Processing (DSP).
12 Extraction of Crude Enzyme.
13 Partial Purification of Enzyme.
14 Precipitation of Enzyme (Salt & Solvent).
15 Dialysis.
16 Characterization of Purified Enzyme.
17 Total Protein Estimation.
18 Bradford's Method.
19 Lowry's Method.
20 Enzyme Assay.
21 Enzyme Kinetics.
22 Effect of pH on Enzyme Activity.
23 Effect of Temperature on Enzyme Activity.
24 Effect of Substrate Concentration on Enzyme Activity.
25 Effect of Activator on Enzyme Activity.
26 Effect of Inhibitor on Enzyme Activity.
27 SDS PAGE.

Techniques Details:(For Duration of 15 Days)

S.No Lab Schedule
1 General and Safety Instructions.
2 Good Laboratory Practices.
3 Principle and Handling of Laboratory Equipments.
4 Basics of Calculations, Weighing and Measurements.
5 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
6 Process of Sterilization and Decontamination.
7 Introduction to Enzymology.
8 Pure Culture Preparation.
9 Screening of Putative Cultures for Enzyme Production.
10 Fermentation (Submerged & Solid State).
11 Downstream Processing (DSP).
12 Extraction of Crude Enzyme.
13 Partial Purification of Enzyme.
14 Precipitation of Enzyme by Solvent Method.
15 Enzyme Assay.
16 Effect of pH on Enzyme Activity.
17 Effect of Temperature on Enzyme Activity.
18 Total Protein Estimation by Bradford's Method.

Module 6

"Hands-on Industrial Training with State of Art Lectures on Recombinant DNA Technology (RDT)"

* (Program Code: CRT-06).

Duration 30 Days / One Month
Seats 15
Fee Structure Rs. 7,000/-

Techniques Details:(For Duration of 30 Days)

S.No Lab Schedule
1 Working in Molecular Biology Laboratory.
2 General and Safety Instructions.
3 Good Laboratory Practices.
4 Principle and Handling of Laboratory Equipments.
5 Basics of Calculations, Weighing and Measurements.
6 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
7 Process of Sterilization and Decontamination.
8 DNA Extraction from Microbes.
9 Purification of Genomic DNA from Several Microbes.
10 Calculation of Yield of Genomic DNA per Bacterium.
11 Determination of Contaminants and Concentration of Genomic DNA by Spectrophotometer.
12 Isolation of Plasmid.
13 Extraction and Purification of Plasmid DNA from E. coli..
14 Electrophoresis of Plasmid DNA.
15 Determination of Purity of Plasmid DNA.
16 Calculation of Yield of Plasmid DNA per Bacterium.
17 Determination of Contaminants and Concentration of Plasmid DNA by Spectrophotometer.
18 Restriction Digestion of Plasmid DNA by Restriction Endonucleases.
19 Separation of Digestion Fragments by Electrophoresis.
20 Construction of Recombinant DNA Molecule.
21 Ligation of Restriction Fragments into Plasmid.
22 Transformation of Recombinant Plasmid in E. coli..
23 Competent Cell Preparation.
24 Transfer of DNA by Heat-Shock Method.
25 Screening of the Transformed Cells.
26 Blue-White Selection of Bacterial Colonies.
27 Analysis of Target Gene.
28 Gene Expression Study in E. coli..
29 Extraction and Purification of Ultra Pure PCR Grade DNA from Various Sources.
30 Determination of Purity of PCR Grade DNA.
31 Determination of Concentration of DNA & Dilution to Unity for PCR.
32 Polymerase Chain Reaction (PCR).
33 Optimization of Protocol for PCR.
34 Principle, Handling & Precautions of PCR Machine.
35 Electrophoresis of PCR Products.

Module 7

"Hands-on Industrial Training with State of Art Lectures on Basic Biotech Techniques i.e. Mixed module"

*(Program Code: CRT-07).

Duration 30 Days / One Month
Seats 15
Fee Structure Rs. 5,200/-

Techniques Details:(For Duration of 30 Days / 1 Month)

S.No Lab Schedule
  Section-A : Bio-Instrumentations.
1 Introduction to Biotechnology and Allied Fields.
2 Introduction to General Lab Rules and Sterilization Techniques.
3 Handling and Operation of Bio-Instruments such as- Digital Weighing Balance, Micro- controller pH Meter, Digital pH Meter, Autoclave, Centrifuge Machine, Distillation Unit, Bacterial Incubator, Hot Air Oven, Water Bath, Shaker Incubator, BOD Incubator, Micro-Pipettes, Vortex Mixer, Magnetic Stirrer, Hot Plate Stirrer, Microscope, Colorimeter, White Light Transilluminator & Laminar Air Flow.
4 Operating Highly Sophisticated Instruments such as Electrophoresis Units (Horizontal & Vertical), Digital UV-Visible PC based Spectrophotometer, Blotting Apparatus, UV Transilluminator, Thermal Cycler (PCR Machine) & Gel Doc. System.
  Section-B : Industrial Microbiology & Fermentation Technology.
5 Basics of Calculations, Weighing and Measurements.
6 Process of Sterilization and Decontamination.
7 Media Preparation.
8 Pouring & Plugging for Bacterial Cultures.
9 Isolation and Culturing of Microbes from Soil Sample (Through Serial Dilution Method).
10 Isolation and Culturing of Microbes from Water Sample (Through Serial Dilution Method).
11 Isolation and Culturing of Microbes from Air (Through Exposure Method).
12 Pure Culture of Microbes through Streaking Method.
13 Slant Preparation & Sub Culturing of Microbes.
14 Staining Techniques.
15 Fermentation (Submerged & Solid State).
16 Downstream Processing (DSP).
  Section-C : Molecular Biology & Proteomics.
17 Working in Molecular Biology Laboratory.
18 General and Safety Instructions.
19 Good Laboratory Practices.
20 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
21 LB-Media Preparation, Inoculation and Culturing of E. coli..
22 Plasmid Isolation from E. coli..
23 DNA Isolation from Plant Sample.
24 Agarose Gel Electrophoresis for Plasmid/Genomic DNA.
25 Restriction Digestion.
26 Isolation of Proteins from Plants and Microbial Systems.
27 Extraction of Total Protein from Various Parts of Plants.
28 Partial Purification of Plant Proteins by Precipitation Method.
29 Total Protein Estimation by Bradford's Method.
30 Paper Chromatography.
31 Thin Layer Chromatography (TLC).

Module 8

"Hands-on Industrial Training with State of Art Lectures on HPLC, PCR & Gel Doc System"

* (Program Code: CRT-08).

Duration 15 Days
Seats 10
Fee Structure Rs. 5,500/-

Techniques Details:(For Duration of 15 Days)

S.No (HPLC)
1 Introduction to Separation Techniques.
2 Introduction to Chromatographic Techniques.
3 Types of Chromatographic Techniques.
4 Explanation of Titrimetric Calculations.
5 Introduction to HPLC Components.
6 Types of Pumps and Functions of their Components.
7 Reservoirs and their Uses.
8 Types of Injectors & their Functions.
9 Types of Detectors & their Functions.
10 Types of Mobile Phase to Separate the Compounds.
11 Types of Stationary Phase to Separate the Compounds.
12 Types of Column.
13 Preparation of Mobile Phase to Separate the Compounds.
14 Method Development for the Separation of Unknown Compounds.
15 How to Select the Mobile Phase.
16 How to Select the Stationary Phase.
17 How to Select the Flow Rate.
18 How to Select the Gradient & Isocratic Methods.
19 How to Select the Sample Volume.
20 How to Select the Wave Lengths or Detectors.
21 Discussions.
(PCR)
1 Extraction and Purification of Ultra Pure PCR Grade DNA from Plants.
2 Electrophoresis of Genomic DNA.
3 Calculation of Yield of DNA per gram of Plant Material.
4 Determination of Purity of PCR Grade DNA.
5 Determination of Concentration of DNA & Dilution to Unity for PCR.
6 Polymerase Chain Reaction (PCR)
7 Optimization of Protocol for PCR.
8 Principle, Handling & Precautions of PCR Machine.
9 Electrophoresis of PCR Products.
10 Discussions.
(Gel Doc System)
1 Principle, Handling & Precautions of Gel Doc.
2 Composed Components & their Functions.
3 Imaging and Documentation of Nucleic Acid and Protein.
4 Applications of Gel Doc System.
5 Discussion.

Module 9

"Hands-on Industrial Training with State of Art Lectures on Specialized Biotech Techniques"

* (Program Code: CRT-09).

THIS IS OUR SECOND MOST EFFECTIVE AS WELL AS JOB ORIENTED MODULE * *

Duration 45 Days 60 Days / Two Months
Seats 15 15
Fee Structure Rs. 10,000/- Rs. 12,000/-

Techniques Details:(For Duration of 45 Days)

S.No Lab Schedule
1 Bio-Instrumentation for Wet Lab..
2 Antibiotics Sensitivity Test.
3 Minimum Inhibitory Concentration (MIC) Test.
4 MDR Test.
5 RNA Isolation from Plant Samples.
6 Denaturing Gel Electrophoresis of RNA.
7 Quantitative Analysis of Nucleic Acid.
8 SDS-PAGE.
9 Blotting Techniques.  (A) Southern/Northern Blotting.  (B) Western Blotting.
10 Extraction and Purification of Plasmid DNA from E. coli..
11 Restriction Digestion.
12 Ligation
13 Competent Cell Preparation.
14 Transformation.
15 Blue-White Screening.
16 Cloning & Gene expression.
17 Extraction and Estimation of Bioactive Compounds from Various Plants.
18 Extraction of Antibiotics from Active Microbes.
19 Downstream Processing (DSP).
20 Salt Precipitation.
21 Dialysis
22 Polymerase Chain Reaction (PCR/Thermal Cycler)
22-A Extraction and Purification of Ultra Pure PCR Grade DNA from Plants.
22-B Electrophoresis of Genomic DNA.
22-C Calculation of Yield of DNA per gram of Plant Material.
22-D Determination of Purity of PCR Grade DNA.
22-E Determination of Concentration of DNA & Dilution to Unity for PCR.
22-F Polymerase Chain Reaction (PCR)
22-G Optimization of Protocol for PCR.
22-H Principle, Handling & Precautions of PCR Machine.
22-I Electrophoresis of PCR Products.
22-J Discussions
23 Gel Documentation System (Gel Doc/Gel Imager)
23-A Principle, Handling & Precautions of Gel Doc.
23-B Composed Components & their Functions.
23-C Imaging and Documentation of Nucleic Acid and Protein.
23-D Applications of Gel Doc System.
23-E Discussion
24 Chromatographic Techniques.
24-A Paper Chromatography.
24-B Thin Layer Chromatography (TLC).
24-C Column Chromatography.
25 High Performance Liquid Chromatography (HPLC)
25-A Introduction to HPLC Components.
25-B Types of Pumps and Functions of their Components.
25-C Reservoirs and their Uses.
25-D Types of Injectors & their Functions.
25-E Types of Detectors & their Functions.
25-F Types of Mobile Phase to Separate the Compounds.
25-G Types of Stationary Phase to Separate the Compounds.
25-H Types of Column.
25-I Preparation of Mobile Phase to Separate the Compounds.
25-J Method Development for the Separation of Unknown Compounds.
25-K How to Select the Mobile Phase.
25-L How to Select the Stationary Phase.
25-M How to Select the Flow Rate.
25-N How to Select the Gradient & Isocratic Methods.
25-O How to Select the Sample Volume.
25-P How to Select the Wave Lengths or Detectors.
25-Q Discussions.

Techniques Details:(For Duration of 60 Days * *)

S.No Lab Schedule
1 Working in Research Laboratory.
2 General and Safety Instructions.
3 Good Laboratory Practices.
4 Principle and Handling of Laboratory Equipments.
5 Basics of Calculations, Weighing and Measurements.
6 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
7 Process of Sterilization and Decontamination.
8 Culturing of Microbes.
9 Types of Culture Media.
10 Media Preparation.
11 Solid and Liquid Media Preparation.
12 Pure Culture Technique.
13 Pour Plate Technique.
14 Spread Plate Technique.
15 Various Streaking Methods.
16 Antibiotics Sensitivity Test.
17 Minimum Inhibitory Concentration (MIC) Test.
18 MDR Test.
19 RNA Isolation from Plant Samples.
20 Denaturing Gel Electrophoresis of RNA.
21 Quantitative Analysis of Nucleic Acid.
22 SDS-PAGE.
23 Blotting Techniques.  (A) Southern/Northern Blotting.  (B) Western Blotting.
24 Extraction and Purification of Plasmid DNA from E. coli..
25 Restriction Digestion.
26 Ligation.
27 Competent Cell Preparation.
28 Transformation.
29 Blue-White Screening.
30 Cloning & Gene expression.
31 Extraction and Estimation of Bioactive Compounds from Various Plants.
32 Extraction of Antibiotics from Active Microbes.
33 Downstream Processing (DSP).
34 Salt Precipitation.
35 Dialysis.
36 Polymerase Chain Reaction (PCR/Thermal Cycler)
36-A Extraction and Purification of Ultra Pure PCR Grade DNA from Plants.
36-B Electrophoresis of Genomic DNA.
36-C Calculation of Yield of DNA per gram of Plant Material.
36-D Determination of Purity of PCR Grade DNA.
36-E Determination of Concentration of DNA & Dilution to Unity for PCR.)
36-F Polymerase Chain Reaction (PCR)
36-G Optimization of Protocol for PCR.
36-H Principle, Handling & Precautions of PCR Machine.
36-I Electrophoresis of PCR Products.
36-J Discussions.
37 Gel Documentation System (Gel Doc/Gel Imager).
37-A Principle, Handling & Precautions of Gel Doc.
37-B Composed Components & their Functions..
37-C Imaging and Documentation of Nucleic Acid and Protein..
37-D Applications of Gel Doc System..
37-E Discussion..
38 Chromatographic Techniques.
38-A Paper Chromatography.
38-B Thin Layer Chromatography (TLC).
38-C Column Chromatography.
39 High Performance Liquid Chromatography (HPLC)
39-A Introduction to HPLC Components.
39-B Types of Pumps and Functions of their Components.
39-C Reservoirs and their Uses.
39-D Types of Injectors & their Functions.
39-E Types of Detectors & their Functions.
39-F Types of Mobile Phase to Separate the Compounds.
39-G Types of Stationary Phase to Separate the Compounds.
39-H Types of Column.
39-I Preparation of Mobile Phase to Separate the Compounds.
39-J Method Development for the Separation of Unknown Compounds.
39-K How to Select the Mobile Phase.
39-L How to Select the Stationary Phase.
39-M How to Select the Flow Rate.
39-N How to Select the Gradient & Isocratic Methods.
39-O How to Select the Sample Volume.
39-P How to Select the Wave Lengths or Detectors.
39-Q Discussions.

Module 10

"Hands-on Industrial Training with State of Art Lectures on Bioprocess Engineering/ Bioprocess Technology/ Fermentation Technology"

* (Program Code: CRT-10).

MOST EFFECTIVE MODULE AS PER INDUSTRIAL DEMAND

Duration 30 Days / One Month 15 Days
Seats 15 15
Fee Structure Rs. 8,500/- Rs. 5,200/-

Techniques Details:(For Duration of 30 Days)

S.No Lab Schedule
1 General and Safety Instructions.
2 Good Laboratory Practices.
3 Principle and Handling of Laboratory Equipments.
4 Basics of Calculations, Weighing and Measurements.
5 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
6 Process of Sterilization and Decontamination.
7 Isolation and Purification of Bacteria from Soil..
8 Screening of Purified Cultures for Amylase Production.  (A) Primary Screening  (B) Secondary Screening
9 Optimization of Physiochemical Factors for Maximum Production of Amylases.
(A) pH
(B) Temperature
(C) Incubation Time
(D) Substrate Concentration
(E) Carbon Source
(F) Nitrogen Source
10 Introduction to Fermentation Processes.
11 Design of Fermenter & Types.
12 Sterilization of Fermenter.
(A) Sterilization of pH probe
(B) Sterilization of DO probe
(C) Sterilization of culture vessel
(D) Sterilization of optimized media
(E) Sterilization of connectors and loops
(F) Sterilization of syringe.
13 Inoculum Development.
14 Instrumentation & Process Control.
(A) Connection of pH probe
(B) Connection of DO probe
(C) Connection of air pump
(D) Optimized pH setting
(E) Optimized DO setting
(F) Optimized temperature setting
15 Production of Amylase.
(A) Submerged fermentation (Batch).
(B) Solid state fermentation.
16 Downstream Processes.
(A) Preparation of Cell Free Extract.
(B) Precipitation of Enzyme.
(C) Dialysis.
17 Determination of Specific activity.
(A) Total activity calculation by DNS Method.
(B) Total Protein estimation by Lowry's Method.
18 Characterization of Purified Enzyme.
(A) pH
(B) Temperature
(C) Activators
(D) Inhibitors
19 Determination of Molecular Weight of Purified Enzyme by SDS PAGE.

Techniques Details:(For Duration of 15 Days)

S.No Lab Schedule
1 General and Safety Instructions.
2 Good Laboratory Practices.
3 Principle and Handling of Laboratory Equipments.
4 Basics of Calculations, Weighing and Measurements.
5 Preparation of Reagents, Stock Solutions & Methods of Labelling and Storage.
6 Process of Sterilization and Decontamination.
7 Isolation and Purification of Bacteria from Soil.
8 Screening of Purified Cultures for Amylase Production.
(A) Primary Screening
(B) Secondary Screening
9 Introduction to Fermentation Processes.
10 Pre-fermentation studies.
(A) Optimization of temperature
(B) Optimization of pH
11 Sterilization of Fermenter.
(A) Sterilization of pH probe
(B) Sterilization of DO probe
(C) Sterilization of culture vessel
(D) Sterilization of optimized media
(E) Sterilization of connectors and loops
(F) Sterilization of syringe
12 Inoculum Development.
13 Instrumentation & Process Control.
(A) Connection of pH probe
(B) Connection of DO probe
(C) Connection of air pump
(D) Optimized pH setting
(E) Optimized DO setting
(F) Optimized temperature setting
14 Production of Amylase by submerged fermentation.
15 Determination of Total Enzyme activity.

Module 11

"Hands-on Industrial Training with State of Art Lectures on Genomics & Proteomics"

*(Program Code: CRT- 11).

Duration 30 Days/One Month
Seats 15
Fee Structure Rs. 7,500/-

Techniques Details:(For Duration of 30 Days)

S.No Section A: (Genomics)
  (PCR)
1 Extraction and Purification of Ultra Pure PCR Grade DNA.
2 Electrophoresis of Genomic DNA.
3 Calculation of Yield of DNA.
4 Determination of Purity of PCR Grade DNA.
5 Determination of Concentration of DNA.
6 Preparation of DNA for PCR.
7 Programming of PCR protocol in Thermal Cycler.
8 Preparation of reaction mixture.
9 DNA amplification.
10 Analysis of PCR products, Spectrophotometrically, Electrophoretically.
11 (Gel Doc System)
11-A Principle, Handling & Precautions of Gel Doc.
11-B Composed Components & their Functions.
11-C Imaging and Documentation of Nucleic Acid and Protein.
11-D Applications of Gel Doc System.
11-E Discussion.
  (Cloning)
12 Restriction Digestion of Vector.
13 Insertion of PCR product into vector.
14 Ligation.
15 Preparation of competent cells.
16 Transformation of ligated vector into competent cells.
17 Blue-White screening.
18 Plasmid Isolation.
19 Discussion.
  Section B: (Proteomics)
20 Fermentation (Submerged & Solid State).
21 Downstream Processing (DSP).
22 Extraction of Crude Enzyme.
23 Partial Purification of Enzyme.
24 Precipitation of Enzyme (Salt & Solvent).
25 Dialysis.
26 Enzyme Assay.
27 Enzyme Kinetics.
27-A Effect of pH on Enzyme Activity.
27-B Effect of Temperature on Enzyme Activity.
27-C Effect of Substrate Concentration on Enzyme Activity.
27-D Effect of Activator on Enzyme Activity.
27-E Effect of Inhibitor on Enzyme Activity.
28 SDS PAGE.

Module 12

"Hands-on Industrial Project/Dissertation work with State of Art Lectures"

*(Program Code: CRT- 12).

Professional Module

Option Duration Seats Fee Structure
CRT-12/1 1 Month 10 Rs. 6,500/-
CRT-12/2 2 Months 10 Rs. 11,000/-
CRT-12/3 3 Months 10 Rs. 16,000/-
CRT-12/4 4 Months 10 Rs. 20,000/-
CRT-12/5 5 Months 10 Rs. 25,000/-
CRT-12/6 6 Months 10 Rs. 30,000/-

NOTE: 25% fee will be charged extra for molecular biology project work

Economical Module

Option Duration Seats Fee Structure
CRT-12/E-1 1 Month 10 Rs. 5,000/-
CRT-12/E-2 2 Months 10 Rs. 8,000/-
CRT-12/E-3 3 Months 10 Rs. 12,000/-
CRT-12/E-4 4 Months 10 Rs. 15,000/-
CRT-12/E-5 5 Months 10 Rs. 18,000/-
CRT-12/E- 6 6 Months 10 Rs. 20,000/-

NOTE: 25% fee will be charged extra for molecular biology project work

NOTE: Candidates (CRT-12) will work on an assigned Live Project for Option-2 to 5. Projects will be related to following areas-

Genetic Diversity Molecular Biology
Industrial Microbiology Enzymology
Plant metabolites Antibiotics
Waste Management Bioremediation
Biochemistry Food Technology
Forensic Science Genetic Engineering
Pharmacology Infection Biology &
  Other high demand areas

&

Biotechnology + Bioinformatics (Wet + Dry Lab) Live Project/ Dissertation Work (3 to 6 Months)

MRD LifeSciencesTM provides a platform to all outstanding candidates who are doing Project/Dissertation work in our lab & help them to publish their papers in National & International Scientific Journals based on their findings of Projects.

Module CBT

"Hands-on Industrial Training with State of Art Lectures on Customized Biotech Techniques"

*(Program Code: CRT- CBT).

Duration One Week/ 7 Days
Seats 15
Fee Structure Rs. 1500/-, 1800/- & 4200/-

Techniques Details:(For Duration of One Week/ 7 Days)

A. Bio-Instrumentation (Fee : Rs. 1,500/-) Introduction to Biotechnology and Allied Fields. Introduction to General Lab Rules and Sterilization Techniques. Handling and Operation of Bio-Instruments such as- Digital Weighing Balance, Micro- controller pH Meter, Digital pH Meter, Autoclave, Centrifuge Machine, Distillation Unit, Bacterial Incubator, Hot Air Oven, Water Bath, Shaker Incubator, BOD Incubator, Micro-Pipettes, Vortex Mixer, Magnetic Stirrer, Hot Plate Stirrer, Microscope, Colorimeter, White Light Transilluminator & Laminar Air Flow. Operating Highly Sophisticated Instruments such as Electrophoresis Units (Horizontal & Vertical), Digital UV-Visible PC based Spectrophotometer, Blotting Apparatus, UV Transilluminator, Thermal Cycler (PCR Machine) & Gel Doc. System.
B. Electrophoretic Techniques (Fee : 1,500/-) Buffer preparation Gel preparation (AGE & SDS PAGE) Sample preparation (DNA & Protein) Electrophoresis Observation of DNA & Protein Bands.
C. PCR & Gel Doc (Fee : Rs. 1,500/-) Preparation of DNA for PCR Programming of PCR protocol in Thermal Cycler Preparation of reaction mixture DNA amplification Analysis of PCR products, Spectrophotometrically, Electrophoretically Gel Documentation.
D. RNA Extraction & Analysis (Fee : Rs. 1,500/-) Preparation of RNAase free plasticwares & glasswares RNA extraction by kit / manual method Analysis of extracted RNA, Denaturing gel electrophoresis, Spectrophotometric method.
E. Bioprocess Technology (Fee : Rs. 1,800/-) Production of target enzyme by shake flask & solid state fermentation Extraction of crude enzyme Purification of crude enzyme, Salt precipitation, Dialysis Assay of purified enzyme.
F. Recombinant DNA Technology (RDT) (Fee : Rs. 1,800/-) Restriction of Genomic DNA & Vector Insertion of insert into restricted vector Ligation Preparation of competent cells Transformation of ligated vector into competent cells. Blue-White screening.
G. HPLC (Fee : Rs. 4,200/-) Introduction to Chromatographic Techniques & HPLC Components. Types of Pumps, Injectors, Detectors & their Functions.Types of Mobile & Stationary Phase to Separate the Compounds.Types of Column. Method Development for the Separation of Unknown Compounds. How to Select the Mobile, Stationary Phase & Flow Rate. How to Select the Gradient & Isocratic Methods. How to Select the Sample Volume. How to Select the Wave Lengths or Detectors.

Module IFT

"Hands-on Industrial Training with State of Art Lectures on Basic & Advanced Industrial Food Technology"

*(Program Code: CRT-IFT).

Duration 30 Days / One Month 15 Days
Seats 20 15
Fee Structure Rs. 7,500/- Rs. 4,500/-

Techniques Details:(For Duration of 30 Days)

S.No Lab Schedule
1 General and Safety Instructions for Working in Food-Tech Lab.
2 Bio-Instrumentation for Food-Tech Lab.
3 Working with Autoclave, Hot-Air Oven, Laminar Air Flow, Microscope and other Food-Tech Instruments.
4 Handling of Micropipettes, Petri plates, Spreaders, Inoculation Loop and other Food-Tech Tools.
5 Media Preparation.
6 Culturing of Microbes.
7 Types of Culture Media.
8 Solid and Liquid Media Preparation.
9 Preparation of Cotton Plug, Plugging for Bacterial Cultures.
10 Sterilization Process (Chemical & Physical Process).
11 Pouring of Media on Plates.
12 Isolation and Preservation of Cultures.
13 Purification of Bacterial Isolates by Streaking Method.
14 Identification of Contaminated Bacteria from Food Sources by Gram Staining Method.
15 Quantification of Microbes: - Sampling and Serial Dilution; Bacterial Counts in Food Products.
16 Microbiological Quality of Milk/ Milk Products.
17 Enumeration and Isolation of E. coli. from Processed Meat/ Chicken.
18 Qualitative Analysis of Carbohydrates.
19 Quantitative Analysis of Carbohydrates.
20 Quantitative Analysis of Protein (Lowry's and Bradford Method).
21 Quantitative Analysis of Lipids (Triglycerides, Cholesterol, Phospholipids, etc.).
22 Determination of Moisture in Spices Powder by Distillation and HAO Method.
23 Determination of Total Fat in Liquid Milk.
24 Determination of Protein Content in Ice-cream.
25 Detection of Adulterants in Edible Oils and Ghee.
26 Detection of Soluble Colour in Spices Powder by TLC.
27 Determination of Vitamin C in Fruit Juices.
28 Determination of Iron Content in Foods.
29 Determination of Lead in Spiced Powder.
30 Estimation of Milk Fat by Gerber Method.
31 Preparation and Analysis of Yoghurt.
32 Analysis of Food Products by Column Chromatography.
33 Qualitative and Quantitative Analysis of Food Products by HPLC.

Techniques Details:(For Duration of 15 Days)

S.No Lab Schedule
1 General and Safety Instructions for Working in Food-Tech Lab.
2 Bio-Instrumentation for Food-Tech Lab.
3 Working with Autoclave, Hot-Air Oven, Laminar Air Flow, Microscope and other Food-Tech Instruments.
4 Handling of Micropipettes, Petri plates, Spreaders, Inoculation Loop and other Food-Tech Tools.
5 Media Preparation.
6 Culturing of Microbes.
7 Types of Culture Media.
8 Solid and Liquid Media Preparation.
9 Preparation of Cotton Plug, Plugging for Bacterial Cultures.
10 Sterilization Process (Chemical & Physical Process).
11 Pouring of Media on Plates.
12 Isolation and Preservation of Cultures.
13 Purification of Bacterial Isolates by Streaking Method.
14 Identification of Contaminated Bacteria from Food Sources by Gram Staining Method.
15 Quantification of Microbes: -Sampling and Serial Dilution; Bacterial Counts in Food Products.
16 Microbiological Quality of Milk/ Milk Products.
17 Qualitative Analysis of Carbohydrates.
18 Quantitative Analysis of Carbohydrates.
19 Quantitative Analysis of Protein (Lowry's and Bradford Method).
20 Determination of Protein Content in Ice-cream.
21 Determination of Vitamin C in Fruit Juices.
22 Determination of Lead in Spiced Powder.
23 Preparation and Analysis of Yoghurt.
24 Qualitative and Quantitative Analysis of Food Products by HPLC.

Module BI

"Hands-on Industrial Training with State of Art Lectures on Basic & Advanced Bioinformatics"

*(Program Code: CRT- BI).

Duration 30 Days / One Month 15 Days
Seats 10 10
Fee Structure Rs. 6,500/-/- Rs. 3,800/-

(BI/A): Advanced Modules (Course Duration 30 Days)

Advanced Bioinformatics (CRT: AM-1)

(Introduction to Bioinformatics and overview of Biological Research, Biological databases and database searching, Data Mining and Sequence Retrieval,Motif & Domain assignment,Proteomics, Protein threading and interaction, Pair wise and Multiple Sequence Alignment, Sequence Analysis (BLAST,FASTA, CLUSTAL W), Phylogenetic analysis, Ab initio structure prediction,Visualization tools, Other relevant Confirmatory Tools, Homology Modeling).

Chemoinformatics and Rational Drug Designing (CRT: AM-2)

(Introduction of Bioinformatics, Introduction to Drug Designing, Disease Identification, Responsible Gene Ontology, Coding Protein Identification, Protein Structure Finding, Active Site Prediction, Chemical and Drug Database, Chemo-informatics, Docking, Toxicity Prediction with Lipinski Rule of Five, Relevant Software and Tools and Practical Sessions).

Molecular Modeling with Phylogenetic Analysis (CRT: AM-3)

(Introduction of Bioinformatics, Literature Mining, Protein Name identification, Target Amino Acid Primary Sequence Identification, Sequence Analysis (Blast, Fasta), Secondary Structure Blast, Multiple Template Selection, Modeling, Procheck, Ramachandran Plot, Loop Modeling, Structure Analysis, Verification and Energy Minimization, Sequence Analysis (Blast, Fasta, ClustalW), Comparative Phylogenetic Analysis using MEGA and Phylip).

Molecular Modeling and Computer Aided Drug Designing (CRT: AM-4)

(Introduction to chemoinformatics and Drug Development, Chemical and Drug Database, Proteomics, Sequence analysis of Receptor molecule, Structural Bioinformatics in Drug Development, Small molecule hits, Designing chemical structures, Homology Modeling Pathways studies and Drug Targeting, Lead design with Geometry optimization and Energy Minimization.

(BI/B): Basic Modules (Course Duration 15 Days)

Bioinformatics (CRT: BM-1)

(Introduction of Bioinformatics, DBMS, Biological Database (NCBI-Genebank, DDBJ, Swissprot-EMBL), File Format, Search Engines and Research Articles Databases, Pairwise and Multiple Sequence Alignment, Phylogenetic Analysis, Sequence Analysis (Blast, Fasta, ClustalW), Secondary Structure Prediction, Biological Visualization Tools).

Phylogenetic Analysis (CRT: BM-2)

(Introduction of Bioinformatics, Pairwise and Multiple Sequence Alignment, Sequence Analysis (Blast, Fasta, ClustalW), Distance Based and Character Based Programs, Comparative Phylogenetic Analysis using Phylogenetic tools).

Biological Data Mining (CRT: BM-3)

(Introduction of Bioinformatics, Nucleotide Sequence Databases (EMBL, Genebank and DDBJ etc.), Protein Sequence Databases (Swissprot, TrEMBL and PIR etc.), Structural Databases (Cath, SCOP, PDB and MMDB etc.) and different biological databases and softwares).

Molecular Modeling (CRT: BM-4)

(Introduction of Bioinformatics, Literature Mining, Protein Name identification, Target Amino Acid Primary Sequence Identification, Sequence Analysis (Blast, Fasta), Secondary Structure Blast, Multiple Template Selection, Modeling, Procheck, Ramachandran Plot, Loop Modeling, Structure Analysis, Verification and Energy Minimization).

Introduction on Drug Designing and Docking Studies (CRT: BM-5)

(Introduction to chemoinformatics and Drug Development, Drug databases and Data Mining, Structural Bioinformatics in drug discovery, Force Fields and Minimizers Concept, Geometry optimization and Energy minimization, Docking, Relevant Softwares).

(BI/C): Core Modules_Mini & Major Projects

(Course Duration 1 Month to 6 Months)

Duration 30 days 45 Days 2 Months 3 Months 4 Months 6 Months
Fee Structure 6500/- 8000/- 10,000/- 15000/- 18000/- 22000/-

Key Research Area :

  • Molecular Modeling with Phylogenetic Analysis
  • Molecular Modeling and Computer Aided Drug Designing
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